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High Resolution Oxygen Measurements

Needle-Type Oxygen Microsensor NTH-PSt7

The glass fiber with its oxygen-sensitive tip (< 50 µm with tapered sensor tip TS, < 230 µm with flat-broken sensor tip TF) is protected inside a stainless steel needle and can be extended for measurements. As long as the sensor tip is retracted and sheltered inside this needle the microsensor can be pierced through septum rubber or any other harsh material. With its small tip size and fast response time (t90 < 3 s) this sensor is ideally suited for research and packaging applications, where micro-invasive and small sensors are needed.

  • High spatial resolution (down to < 50 μm)
  • High temporal resolution (t90 < 3 sec.)
  • No consumption of oxygen
  • Signal independent of flow velocity
  • Measures in liquids as well as in gas phase
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Applications

Tissue Engineering

Oxygen microsensors measure the oxygen content in various volume compartments of the tissue engineering constructs. To do so, hair-thin sensors are inserted into the constructs and the oxygen content is measured online. In this way, the oxygen partial pressure is measured with a high local resolution and correlated with the constructs tissue quality (composition of the extracellular matrix).


Packaging & Quality Control

Oxygen inside packaging can lead to oxidative deterioration of certain products. Therefore, determination of the oxygen content within packages or pharmaceutical vials is of essential importance to ensure both the filling quality and the long-term storage stability. With our micro-invasive needle-type oxygen microsensors we offer a simple tool to determine residual oxygen both in the headspace and in liquids. The septum of the vial or package is pierced with the needle and the sensor is extended for measurement. As the measurement is made inside the package no error-prone and time consuming sampling is necessary. These oxygen microsensors are ideal tools for quality control in the food & beverage, packaging, and pharmaceutical industries.


Technical

Specifications* Gaseous & Dissolved O2 Dissolved O2
*data for microsensor TF = flat-broken sensor tip with 230 μm diameter
**after two-point calibration as described in the manual
Measurement range 0 – 100 % O2
0 – 1000 hPa

0 – 45 mg/L

0 – 1400 µmol/L
Limit of detection 0.03 % oxygen 15 ppb
Resolution

± 0.01 % O2 at 1  % O2
± 0.05 % O2 at 20.9 % O2

± 0.005 mg/L at  0.4mg/L

± 0.025 mg/L at 9.06 mg/L
Accuracy at + 20 °C** ± 0.05 % O2 or ± 3 % rel.
Measurement temperature range from 0 to + 50 °C
Response time (t90) < 3 sec. (gas) < 10 sec. (liquid)
Properties    
Compatibility Aqueous solutions, ethanol, methanol
No cross-sensitivity

pH 1 – 14
CO2, H2S, SO2
Ionic species

Cross-sensitivity

Organic solvents, such as acetone, toluene, chloroform or methylene chloride
Chlorine gas

Sterilization procedure

Ethylene oxide (EtO)

Cleaning procedure

3 % H2O2
Ethanol
Soap solution

Calibration Two-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and air-saturated environment
Storage stability 60 months provided the sensor material is stored in the dark at room temperature

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Resources

Publications

Oxygen Consumption of Crayfish under Different Temperature Regimes Alginate-based Bi-layered Hydrogels with Embedded Cells for the Regenerative Therapy of Osteo-chondral Defects Hypoxic Preconditioning of Human Mesenchymal Stem Cells Hypoxia in Static and Dynamic 3D Culture Systems New mL-Scale Bioreactor for the Cultivation of Mycelium Forming Microorganisms Hydrogels as Cell Carriers for Tissue Engineering Alternative oxidase plays a role in minimizing ROS and RNS produced under salinity stress in Arabidopsis thaliana Phytoglobin-NO cycle and AOX pathway play a role in anaerobic germination and growth of deepwater rice The Ostrea chilensis pallial cavity: nursery, prison, and time machine The gaseous gastrointestinal tract of a seawater teleost, the English sole (Parophrys vetulus) “Sinking dead”—How zooplankton carcasses contribute to particulate organic carbon flux in the subantarctic Southern Ocean Metabolic response to increasing environmental temperature in the invasive mussel Limnoperna fortunei Introducing Candidatus Bathyanammoxibiaceae, a family of bacteria with the anammox potential present in both marine and terrestrial environments Oxygen transfer of microbubble clouds in aqueous solutions – Application to wastewater Substrate-Specific Coupling of O2 Activation to Hydroxylations of Aromatic Compounds by Rieske Non-heme Iron Dioxygenases Elimination of Induced Hypoxic Regions in Depth of 3D Porous Silk Scaffolds by the Introduction of Channel Configuration Characterization of a simple 'hanging bag' Photobioreactor for low-cost cultivation of microalgae Profound alterations in brain tissue linked to hypoxic episode after device implantation Local gas transfer rate through the free surface in spatially accelerated open-channel turbulence Influence of oxygen deficiency and the role of specific amino acids in cryopreservation of garlic shoot tips Effects of hypoxic preconditioning on neuroblastoma tumour oxygenation and metabolic signature in a chick embryo model Studies on gas exchange in the meadow spittlebug, Philaenus spumarius: the metabolic coast of feeding on, and living in, xylem sap Hyaluronic acid facilitates chondrogenesis and matrix deposition of human adipose derived mesenchymal stem cells and human chondrocytes co-cultures Temperature-dependent stress response in oysters, Crassostrea virginica: Pollution reduces temperature tolerance in oysters Response of Aquatic Leaf Associated Microbial Communities to Elevated Leachate DOC: A Microcosm Study The role of singlet oxygen and oxygen concentration in photodynamic inactivation of bacteria Degradation and mineralization of coral mucus in reef environments Hypoxic life of intertidal acorn barnacles Saving by freezing? Metabolic rates of Adamussium colbecki in a latitudinal context Development, Parallelization and Automation of a Gas-Inducing Milliliter-Scale Bioreactor for High-Troughput Bioprocess Design (HTBD) Rapid Depletion of Dissolved Oxygen in 96-Well Microtiter Plate Staphylococcus epidermidis Biofilm Assays Promotes Biofilm Development and Is Influenced by Inoculum Cell Concentration Essential fatty acids influence metabolic rate and tolerance of hypoxia in Dover sole (Solea solea) larvae and juveniles Low oxygen sensing and balancing in plant seeds: a role for nitric oxide Sulfide-iron interactions in domestic wastewater from a gravity sewer New Milliliter-Scale Stirred Tank Bioreactors for the Cultivation of Mycelium Forming Microorganisms Effective diffusion and microbiologic activity as constraints describing pyrite oxidation in abandoned lignite mines Rising groundwater tables in partly oxidized pyrite bearing dump-sediments: Column study and modelling approach Differences in rhizome aeration of Phragmites australis in a constructed wetland Oxygen requirements of zebrafish (Danio rerio) embryos in embryo toxicity tests with environmental samples Online oxygen measurements in ex vivo perfused muscle tissue in a porcine model using dynamic quenching methods Migration capacity and viability of human primary osteoblasts in synthetic three-dimensional bone scaffolds made of tricalciumphosphate Neutralization by Metal Ions of the Toxicity of Sodium Selenide Symbiotic Leghemoglobins Are Crucial for Nitrogen Fixation in Legume Root Nodules but Not for General Plant Growth and Development Bark and woody tissue photosynthesis: a means to avoid hypoxia or anoxia in developing stem tissues In situ metabolism in halite endolithic microbial communities of the hyperarid Atacama Desert

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